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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Cold Spring Harb Protoc. 2015 Mar 11;2015(7):pdb.prot083659. doi: 10.1101/pdb.prot083659

FIGURE 1.

FIGURE 1

Common scheme for hybridization-based whole-exome enrichment methods. In summary, genomic DNA is fragmented, denatured, and hybridized with capture oligos during library preparation for high-throughput sequencing. The captured sequences are then enriched with streptavidin-conjugated paramagnetic beads and further amplified before being subjected to Illumina sequencing. Diagrammed DNA sequences in red, on-target regions; sequences in blue, off-target regions; single-stranded oligos in green, capture probes labeled with biotin; brown particles, streptavidin-conjugated paramagnetic beads.