(A–C) Primary peritoneal macrophages obtained from C57BL/6J WT (black columns) or
MyD88-deficient (open columns) female mice were kept in cell culture overnight and
challenged with PR8 at 1 MOI for 2 hours. Cytokine content was measured in supernatants 24
hours post infection.
(D) Survival of mice infected with PR8. C57BL/6J female mice received intranasal
inoculate of PR8 (~7500 TCID50; ~ LD90), on day “0”.
Starting from day 2, the mice received 2R9, CP, Eritoran (E5564), or vehicle once daily
for the course of 5 successive days. Peptides were administered i.p. at the dose 200 nmol.
Eritoran was administered i.v. at the dose 200 μg (Shirey et al., 2013).