Skip to main content
. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Cell Rep. 2015 Jun 18;11(12):1941–1952. doi: 10.1016/j.celrep.2015.05.035

Figure 6. 2R9 suppresses PR8-induced cytokine secretion by macrophages and improves survival in a mouse model of influenza.

Figure 6

(A–C) Primary peritoneal macrophages obtained from C57BL/6J WT (black columns) or MyD88-deficient (open columns) female mice were kept in cell culture overnight and challenged with PR8 at 1 MOI for 2 hours. Cytokine content was measured in supernatants 24 hours post infection.

(D) Survival of mice infected with PR8. C57BL/6J female mice received intranasal inoculate of PR8 (~7500 TCID50; ~ LD90), on day “0”. Starting from day 2, the mice received 2R9, CP, Eritoran (E5564), or vehicle once daily for the course of 5 successive days. Peptides were administered i.p. at the dose 200 nmol. Eritoran was administered i.v. at the dose 200 μg (Shirey et al., 2013).