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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Pharm Res. 2015 Feb 12;32(8):2548–2558. doi: 10.1007/s11095-015-1643-0

Fig. 3.

Fig. 3

Cytotoxicity assay on 4T1 cells after incubating with citral/NP, neral/NP and geranial/NP for 72 h (A). At the highest concentrations of drug tested, empty NP were not toxic to cells with >90% cell viability, data not shown on graph. The IC50 for citral/NP, neral/NP and geranial/NP was calculated to be 4.5, 9.9, and 1.4 μM respectively by curve fitting with Graph Pad Prism 5 Software. After 24 h incubation, western blot reveals increased expression of Atg5 (55k Da) and LC3B (14 kDa) proteins in 4T1 cells treated with 25 μM of each formulation, indicative of autophagy (B).