Figure 2. Palmatine inhibits anchorage-independent growth in vitro.

Percent colonies formed by DU145 (A), PC-3 (B), LNCaP (C) and C4-2B (D) cells after palmatine treatment and incubation for 7 days on soft agar. At end-point, agar was solubilized, cells lysed and fluorescence detected using CyQuant GR dye using a plate reader. Data show mean values ± SD for 2 independent experiments. *p < 0.05; **p < 0.01;.