Figure 6.
Nitric oxide increases wild-type (WT) lymphatic vessel permeability. Lymphatic vessel permeability to albumin (PsBSA) was assessed before and after manipulation of nitric oxide signalling. (A) The basal permeability of collecting lymphatics was decreased after application of a pan-inhibitor of nitric oxide synthase (l-NAME, 100 μmol/L). (B) Genetic deletion of eNOS resulted in a similar decrease in collecting lymphatic permeability. (C) Exposure to a nitric oxide donor (SNP, 1 μmol/L) resulted in an increase in lymphatic permeability. (D) Histamine (10 μmol/L) evoked endogenous nitric oxide production that also led to an increase in collecting lymphatic permeability. *Significantly different from control (P < 0.05). Each panel represents n = 6 to 7 vessels from at least six mice.