Figure 7.

Phosphorylation of RyR2 and CaMKII activity. (A) Representative blots of phosphorylation and total levels of RyR2; (B and C) Percentage of phosphorylated Ser2808 (pSer2808) and Ser2814 (pSer2814) RyR2 in infected cardiomyocytes (n = 6 hearts); (D) CaMKII activity in GFP, WT, and R25C cardiomyocytes (n = 7 hearts); (E) Representative blots of phosphorylation and total levels of CaMKII; (F) Percentage of phosphorylated Thr286 (pT286) CaMKII in infected cardiomyocytes (n = 4 hearts); (G and H) Ca²⁺ spark frequency and percentage of Ca²⁺ waves recorded in GFP, WT, and R25C cardiomyocytes in the absence or presence of CaMKII inhibitor KN93 (1 µmol/L), with KN92 (1 µmol/L) used as a control (15–20 cells were measured per experiment or each heart; n = 4 hearts for GFP, WT-PLN, and R25C-PLN groups). *P < 0.05, vs. WT-Basal, ^†P < 0.05, vs. R25C-Basal. Values are mean ± SE.