Table 3.
Primers | Sequence |
---|---|
ssp Pro For EcoRI | 5′-TAGCGAATTCGATATGTTGAACT GGACGTCGTGAAC-3′ |
ssp Pro Rev XmaI | 5′-TTCCCGGGCTAAAAACCTCCAA AAAATTTATTTACAAGTTAAATA TAACAC-3′ |
sspB-For | 5′-AGGAGGTTTAAACTATGAATAGTTCATA TAAATCTAGAGTATTCA ATATTATAAGC-3′ |
sspB-Rev-AscI | 5′-TTGGCGCGCCTTAGTAACC TATCATTGAACCATACCAG-3′ |
sspABC-For | 5′-AGGAGGTTTAAACTATGAAAGGT AAATTTTTAAAAGTTAGTTC TTTATTCG-3′ |
sspABC-Rev-AscI | 5′-TT GGCGCGCCTTATACTAAGCG CTCATAAACGATTGG-3′ |
AirROE-for | 5′-AAACTATGGAACAAAGG ACGCGAC-3′ |
AirROE-rev | 5′-TTGGCGCGCCCTATTTTA TAGGAATTGTGAATTG-3′ |
pJB38-ssp For | 5′-GAATATGATATTAAGTC ACTTGCGTCG-3′ |
pJB38-ssp Rev | 5′-GCTTATGAAATGGATGTT TTAAAAGAAGGTATG-3′ |
luxArev | 5′-GCT CCA GTA ACC ATA CGG TAT C- 3′ |
TetRfor | 5′-CAA TAC AAT GTA GGC TGC -3′ |
TTrev | 5′-CTC AGG AGA GCG TTC AC -3′ |
aPurchased from IDT DNA, Coralville, IA.