Figure 5. Effect of isocryptotanshinone (ICTS) on the mitogen activated protein kinases signaling. (A) MCF-7 cells were treated with indicated concentrations of ICTS for 24 hours, and the expressions of total and phosphorylated JNK, ERK, p38 were detected by Western blot. Glycer-aldehyde 3-phosphate dehydrogenase (GAPDH) was used as internal control. (B) Cells were treated with ICTS for 0, 1, 3, 6, 12, and 24 hours, respectively, the expressions of p-JNK, p-ERK, and p-p38 were detected by Western blot. GAPDH was used as internal control.
