Fig. 3.

Treatment with cAMP-elevating agents increases Ca²⁺ influx. A: the effects of forskolin/H-89 treatment on Ca²⁺ influx in larval zebrafish. B: the effects of 8-bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP) treatment on Ca²⁺ influx in larval zebrafish. C: the effects of isobutylmethylxanthine (IBMX) treatment on Ca²⁺ influx in larval zebrafish. In all experiments, fish were acclimated to low-[Ca²⁺] (25 μM) water beginning at 1 day postfertilization (dpf), and influx was performed in normal [Ca²⁺] (250 μM) water at 4 dpf. Values are means ± SE; N = 6. ^a,b,c Different letters represent a statistical difference from each other (one-way ANOVA, followed by a Holm-Sidak post hoc test; P < 0.05). *Statistical difference (Student's t-test, P < 0.05).