Fig. 4.

Inhibition of p38 MAP kinase reduces the impact of histamine on lamellipodia protrusion and withdrawal. A: time courses of lamellipodia PF, PD, PP, and PV during baseline, addition of 6 μM SB203580, and addition of 10 μM histamine. B: time course changes in lamellipodia WD, WT, and WV, along with the percentage of protrusions with a WT greater than 5 min during baseline, SB203580 (SB) treatment both before and after histamine was added. C: maximum changes in the aforementioned protrusion and withdrawal parameters are directly compared between cells that were treated with histamine in the absence or presence of SB203580. *P < 0.05, in A compared with baseline (−20 min time point), and in C between the two groups. Measurements came from n = 9 cells from 3–4 different experiments in each group.