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. 2015 Jun 16;6:7451. doi: 10.1038/ncomms8451

Figure 6. Absence of D antigen-mediated agglutination in RHD-knockout cell lines.

Figure 6

Parental, RHD-knockout (E1_B, E4_B) and RHD-monoallelic mutant (E4_M) HiDEP-1 cells were induced for differentiation for 4 days and subjected to an agglutination test using anti-D blood grouping reagents (a) and a weak D test using anti-D blood grouping reagents and Coombs' reagent (Anti-IgG, -C3d) (b) in 96-well plates and on glass slides. Rh D-positive, D-negative and weak D-positive human peripheral blood cells were used as the controls. A photograph and photomicrographs of each cell line are shown. Scale bar, 500 μm.