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Glutathione-S-transferase (GST)-14-3-3 pulldown assay. UT-A1 Madin-Darby canine kidney cells pretreated with 10 μM forskolin (FSK) for 0, 5, 15, and 30 min. Cell lysates were prepared for GST-14-3-3 precipitation and detected by immunoblot analysis (IB) with urea transporter (UT)-A1 antibody. The same membranes were stripped and reprobed with pan-14-3-3 antibody. *Unknown nonspecific bands.
