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Effect of 14-3-3γ coexpression on UT-A1 urea transport activity. Oocytes were coinjected with cRNAs encoding UT-A1 (2 ng/cell) alone or with 14-3-3γ or 14-3-3σ (5 ng/cell) for 3 days. A: urea transport activity was measured by 14C-labeled urea flux (n = 5∼6 oocytes/3 min). **P < 0.01. B: 10 oocytes from each group were lysed in RIPA buffer. Protein expression was examined by Western blot analysis with UT-A1, pan-14-3-3, and actin antibodies.
