Figure 2. Performance of SM_SN level detection of DNA methylation.

Multiple metrics showing the performance of the proposed single molecule, single nucleotide (SM_SN) detection method. (a) Performance of the approach for detecting 6mA modifications in the 5′-CTGCAG motif of E. coli C227 using three thresholds for minimum single-molecule coverage (cov_SM). (b) Distribution of the aggregate, single nucleotide (Agg_SN) and SM_SN methylation scores for the partially non-methylated 5′-RGATCY motif in C. salexigens. The bimodal distribution of the SM_SN scores enables the accurate and objective estimation of this fraction. (c) Accuracy of SM_SN-enabled estimations of the methylated fraction (using cov_SM≥10) for the 5′-CTGCAG motif of E. coli C227 at various levels of genomic-sequencing coverage.