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. 2015 May 26;16(6):11966–11982. doi: 10.3390/ijms160611966

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of the proliferative abilities of sorted CD44⁺/CD24⁻ and CD44⁺/CD24⁺ subpopulations of MCF-7 cells and Mucin1(MUC1) protein levels in MCF-7 cells cultured in four types of media. (A) 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay examining the proliferative ability of the CD44⁺/CD24⁻ cells of the three groups. Results are typical of the three independent experiments. Data represent means ± S.E. (n = 3). *** p < 0.001 vs. the normal media group; (B) MTS assay examining the proliferative ability of the CD44⁺/CD24⁺ cells of the three groups. Results are typical of the three independent experiments. Data represent means ± S.E. (n = 3); (C) Western blot analysis of MUC1 levels. Band intensity was analyzed using Quantity One software and β-actin was used as a loading control. Protein levels were compared to the normal media group. Results are typical of three independent experiments. Data represent means ± S.E. (n = 3). * p < 0.05, vs. the Normal media group; ^# p < 0.05 vs. the Mac group; ns means no significance.

Analysis of the proliferative abilities of sorted CD44⁺/CD24⁻ and CD44⁺/CD24⁺ subpopulations of MCF-7 cells and Mucin1(MUC1) protein levels in MCF-7 cells cultured in four types of media. (A) 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay examining the proliferative ability of the CD44⁺/CD24⁻ cells of the three groups. Results are typical of the three independent experiments. Data represent means ± S.E. (n = 3). *** p < 0.001 vs. the normal media group; (B) MTS assay examining the proliferative ability of the CD44⁺/CD24⁺ cells of the three groups. Results are typical of the three independent experiments. Data represent means ± S.E. (n = 3); (C) Western blot analysis of MUC1 levels. Band intensity was analyzed using Quantity One software and β-actin was used as a loading control. Protein levels were compared to the normal media group. Results are typical of three independent experiments. Data represent means ± S.E. (n = 3). * p < 0.05, vs. the Normal media group; ^# p < 0.05 vs. the Mac group; ns means no significance.