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. 2015 Jun 1;16(6):12345–12359. doi: 10.3390/ijms160612345

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Morphological differences between wild-type (WT) and Nf1 haploinsufficient (Nf1^+/−) mesenchymal stem/progenitor cells (MSPCs). (A) Morphology of WT and Nf1^+/− MSPCs imaged under 200× amplification by phase contrast microscopy. Cells were stained with 400 nM fluorescein isothiocyanate(FITC)-phalloidin and DAPI; (B) A quantitative comparison of nuclear-cytoplasmic ratio between WT and Nf1^+/− MSPCs based on the average ratio of nuclear area/cytoplasm area in 50 cells/field from five different fields. Data are represented as mean ± SD from three batches of MSPCs isolated from individual mice (* p < 0.05 for Nf1^+/− vs. WT MSPCs).