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. 2015 Jun 1;16(6):12345–12359. doi: 10.3390/ijms160612345

Figure 3.

Figure 3

Enhancement of cellular adhesion in Nf1+/− MSPCs. (A) MSPCs were plated into wells pre-coated with either 8 µg/mL CH296 (recombinant fibronectin fragment) or 0.1% bovine serum albumin (BSA). Following a 30 min incubation period at 37 °C, the plates were washed and adherent cells were counted on five representative fields/well from six replicate wells, (original magnification ×200); (B) Nf1+/− MSPCs had significantly increased adhesion to CH296 coated plates in comparison to WT MSPCs (*** p < 0.001 for CH296 coated Nf1+/− MSPCs vs. CH296 coated WT MSPCs); (C) Preferential adhesion to fibronectin binding sites, H296 and CH271, was assessed. Nf1+/− MSPCs exhibited significantly greater adhesion to CH271 as compared to H296. (*** p < 0.001 for CH271 coated Nf1+/− MSPCs vs. CH271 coated WT MSPCs); (D) Expression of CH271 receptor, CD49e, was quantified by flow cytometry, demonstrating significantly increased expression of CD49e in Nf1+/− MSPCs in comparison to WT. The green lines represent isotype controls while the green solid areas represent the experimental samples. Data are one representative result of three independent experiments, and each experiment was performed with different MSPCs culture isolated from individual mice.