Figure 4.

Nf1^+/− MSPCs exhibit increased Akt (also known as protein kinase B) and extracellular-signal-regulated protein kinase (Erk)1/2 phosphorylation, which can be inhibited by LY294002 and PD0325901, respectively. Phosphorylation of Akt and Erk1/2 was determined by Western blot in WT and Nf1^+/− MSPCs following 10% FBS stimulation in the presence or absence of PI3-K inhibitor, LY294002, or MAPK inhibitor, PD0325901. Data represents one of three independent experiments, and each experiment was performed with different MSPCs culture isolated from individual mice.