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Mouse mAbs Ex14-1 (A) and 1CKLH18 (B) specifically detected endogenous sFlt1 isoforms from amniotic fluid on a Western blot. Each mAb recognized a single protein of the expected molecular weight (~115 kDa) when compared to recombinant standards. Included as a positive control (C) is a commercially available mAb (Sigma Cat.#V4262) that recognized total sFlt1.
