. 2015 Jun 3;16(6):12513–12530. doi: 10.3390/ijms160612513

Table 1.

Selected candidate reference genes, primer sequences and PCR amplification characteristics.

Gene/GenBank Accession Number	Description	Function	Forward (F) and Reverse (R) Primer Sequences (5′→3′)	Amplicon Length	Tm (°C)	Amplification Efficiency (%)	Correlation Coefficient
18S/KP729648	18S ribosomal RNA	ribosomal structure	F: ACCAGGTCCAGACATAGTAAGGATTGA	140 bp	81.73	106.40	0.999
18S/KP729648	18S ribosomal RNA	ribosomal structure	R: AGTTAGCAGGCTGAGGTCTCGTT	140 bp	81.73	106.40	0.999
ACT/GADC01011038	actin	cytoskeletal structural protein	F: CCAGAAGTCTTGTTCCAGCCATCTC	185 bp	80.66	105.78	0.999
ACT/GADC01011038	actin	cytoskeletal structural protein	R: GCGGTGATCTCCTTGCTCATACG	185 bp	80.66	105.78	0.999
CYC/GADC01018836	cyclophilin	protein folding	F: GGCAAGATACGAACGGATCACAGTT	145 bp	82.95	108.93	0.999
CYC/GADC01018836	cyclophilin	protein folding	R: GGCACTCCACTCCGACTTCCTT	145 bp	82.95	108.93	0.999
EF1α/GADC01006492	elongation factor 1-alpha	protein biosynthesis	F: GGTATTCTCAAGCCTGGTATGGTTGT	102 bp	80.48	94.98	0.999
EF1α/GADC01006492	elongation factor 1-alpha	protein biosynthesis	R: GAGAGCCTCCTGAAGAGCCTCAT	102 bp	80.48	94.98	0.999
GAPDH/GADC01052274	glyceraldehyde-3-phosphate dehydrogenase	glucose metabolism	F: TGGCAAGCATATTCAGGCAGGAG	116 bp	81.63	94.98	0.999
GAPDH/GADC01052274	glyceraldehyde-3-phosphate dehydrogenase	glucose metabolism	R: TTGGCTCATCAGGATTGTAGGTATCAG	116 bp	81.63	94.98	0.999
PLA/KP729647	phospholipase A22	lipid catabolic process	F: ATACCATACAGAACGCAGCTTGTGAA	101 bp	79.92	103.33	0.998
PLA/KP729647	phospholipase A22	lipid catabolic process	R: TTCCGCCAGTTCCAACCTATCCA	101 bp	79.92	103.33	0.998
RPII/GADC01020629	RNA polymerase II subunit	mRNA process	F: GCCTCGGTCTCATTCCTCTTACAAG	109 bp	82.44	104.17	0.999
RPII/GADC01020629	RNA polymerase II subunit	mRNA process	R: AACTCAACAGAACAATACTCGCACTGA	109 bp	82.44	104.17	0.999
RPS13/GADC01008223	30S ribosomal protein S13	DNA-templated transcription	F: TAATGCACAGCTTCCAGATGAC	202 bp	81.47	90.55	0.999
RPS13/GADC01008223	30S ribosomal protein S13	DNA-templated transcription	R: AACCAGTCGCTTTGATTCTTCT	202 bp	81.47	90.55	0.999
TEF2/GADC01000224	transcription elongation factors-II	transcription	F: AGATTCAGAGCATGAAGAGGGAC	182 bp	82.18	104.17	0.996
TEF2/GADC01000224	transcription elongation factors-II	transcription	R: CGATCGGTATTTGTTGCGATTT	182 bp	82.18	104.17	0.996
TUB/GADC01018931	Tubulin beta-4 chain	structural constituent of cytoskeleton	F: ACAATTCACTGCCATGTTCAGGAGAA	169 bp	82.05	97.83	0.999
TUB/GADC01018931	Tubulin beta-4 chain	structural constituent of cytoskeleton	R: GTCATCTTCGTAGTCACCTTCGTCATC	169 bp	82.05	97.83	0.999
UBL/GADC01024109	ubiquitin-like	protein binding	F: GCTACGTCTGCGTGGAGGAATG	197 bp	82.39	99.53	0.996
UBL/GADC01024109	ubiquitin-like	protein binding	R: TGTAGTCTGCCAATGTGCGTCC	197 bp	82.39	99.53	0.996
UCE/GADC01034781	ubiquitin-conjugating enzyme	ubiquitin-dependent protein catabolic process	F: TGGAATGGATGACGGAGACGACAT	142 bp	78.74	100	0.997
UCE/GADC01034781	ubiquitin-conjugating enzyme	ubiquitin-dependent protein catabolic process	R: AACACTTGGTGGCTTCTCTGGATAATC	142 bp	78.74	100	0.997