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. 2015 Jun 11;16(6):13356–13380. doi: 10.3390/ijms160613356

Figure 5.

Figure 5

BAPTA-AM blocks mitophagy in YFP-Parkin RPE1 cells. (A) Establishing the shortest CCCP treatment for a robust mitophagy response; (B) Example images of mitochondrial content in YFP-Parkin RPE1 cells treated with CCCP in the absence or presence of 10 µM EGTA-AM or BAPTA-AM, followed by a 21-h wash; (C) Quantitation of mitophagy in cells treated with EGTA-AM or BAPTA-AM using the same treatment-chase procedure (3-h treatment; 21-h wash); (D) Measurement of the autophagy (LC3 lipidation) response in cells treated with 5 µM or 10 µM BAPTA-AM in the absence or presence of CCCP; (E) Live cell imaging of YFP-Parkin recruitment in RPE1 cells transfected with Mito-dsRed in the absence or presence of 5 µM BAPTA-AM, treated with CCCP. * p < 0.05; *** p < 0.001 one-way ANOVA, Tukey’s post-test. Scale bars = 10 µm.