Table 1.
Summary of the autophagy and mitophagy screens.
| Gene | Gene ID | siRNA Cat. No | Autophagy Screen | Mitophagy Inhibited? | ||
|---|---|---|---|---|---|---|
| ATG5 | LC3 | Defect? | ||||
| DNM1L | 10059 | M-012092-01 | 0.6 | 0.7 | No | No |
| HSPA9 | 3313 | M-004750-03 | 1.4 | 1.05 | No | No |
| MFF | 56947 | M-018261-01 | 1.7 | 0.6 | No | No |
| MARCH5 | 54708 | M-007001-0 | 0.65 | 0.6 | No | No |
| FIS1 | 51024 | M-020907-02 | 0.85 | 0.3 | No | No |
| MFN1 | 55669 | M-010670-01 | 1.0 | 0.4 | No | No |
| MFN2 | 9927 | M-012961-00 | 1.0 | 0.15 | No | No |
| RHOT1 | 55288 | D-010365-01 | 0.85 | 0.85 | No | ND |
| RHOT2 | 89941 | M-008340-01 | 0.65 | 0.4 | No | ND |
| BCAP31 | 10134 | M-018679-00 | 1.4 | 0.1 | No | No |
| PACS2 | 23241 | M-022015-01 | 0.65 | 0.3 | No | No |
| TRAK1 | 22906 | M-020331-02 | 0.8 | 0.5 | No | ND |
| TRAK2 | 66008 | M-014141-00 | 1.2 | 0.4 | No | ND |
| VAPB | 9217 | M-017795-00 | 1.85 | 0.35 | Yes | No |
| STX18 | 53407 | M-020624-00 | 1.05 | 0.05 | No | No |
| VDAC1 | 7416 | M-019764-00 | 0.95 | 0.25 | No | No |
| IP3R1 | 3708 | M-006207-01 | 0.3 | 0.85 | No | No |
| IP3R2 | 3709 | M-006208-01 | 0.55 | 0.55 | No | No |
| IP3R3 | 38710 | M-006209-02 | 0.55 | 0.8 | No | No |
| ATF6 | 22926 | M-009917-01 | 1.05 | 0.55 | No | No |
| EIF3AK3 | 9451 | M-004883-03 | 0.55 | 0.7 | No | No |
| ERN1 | 2081 | M-004951-02 | 0.5 | 1.1 | No | No |
| ANK2 | 287 | M-008417-02 | 0.65 | 1.4 | No | No |
| ATP2A1 | 487 | M-006113-00 | 1.4 | 0.3 | No | No |
| ATP2A2 | 488 | M-004082-01 | 1.9 | 0.4 | Yes | No |
| ATP2A3 | 489 | M-006114-01 | 0.85 | 0.75 | No | No |
| RMDN3 | 55177 | D-020973-01 | 0.7 | 0.35 | No | No |
| TMBIM6 | 7009 | M-004118-01 | 0.9 | 2.1 | No | No |
| ESYT1 | 23344 | M-010652-00 | 1.3 | 1.35 | No | No |
| ESYT2 | 57488 | M-025231-00 | 0.8 | 1.35 | No | No |
| ESYT3 | 83850 | M-023602-01 | 0.55 | 0.45 | No | No |
| SIGMAR1 | 10280 | M-017475-02 | 2.3 | 0.2 | Yes | No |
| PEMT | 10400 | D-010392-01 | 0.85 | 0.8 | No | Yes |
| PISD | 23761 | M-009548-00 | 1.6 | 0.85 | No | No |
| PTDSS1 | 9791 | M-008568-00 | 0.65 | 0.4 | No | No |
| PTDSS2 | 81490 | M-008959-00 | 0.95 | 0.7 | No | No |
| TEX2 | 55852 | M-017117-00 | 0.9 | 1.15 | No | No |
| ATL2 | 64225 | M-014047-00 | 0.65 | 0.65 | No | No |
| ATL3 | 25923 | M-010656-00 | 0.15 | 1.35 | No | No |
| CKAP4 | 10970 | M-012755-01 | 1.5 | 0.25 | Yes | No |
| KTN1 | 3895 | M-010605-01 | 0.55 | 0.95 | No | No |
| REEP1 | 65055 | M-014235-01 | 1.45 | 0.75 | No | No |
| REEP2 | 51308 | M-021201-00 | 0.85 | 0.2 | No | No |
| REEP3 | 221035 | M-032301-0 | 0.85 | 0.9 | No | No |
| REEP5 | 92840 | M-019467-0 | 0.4 | 0.3 | No | No |
| REEP6 | 7905 | M-015555-00 | 0.4 | 0.3 | No | No |
| RTN1 | 6252 | M-014138-00 | 1.4 | 1.1 | No | No |
| RTN2 | 6253 | M-012717-00 | 0.95 | 0.35 | No | No |
| RTN3 | 10313 | M-020088-00 | 0.9 | 1.1 | No | No |
| RTN4 | 57142 | M-010721-00 | 1.1 | 0.45 | No | No |
| PSEN2 | 5664 | M-006018-02 | 1.1 | 0.55 | No | No |
| LRPPRC | 10128 | M-018773-00 | 0.45 | 0.75 | No | No |
| MAPRE1 | 22919 | M-006824-00 | 1.0 | 1.3 | No | No |
| PDZD8 | 118987 | M-018369-01 | 0.85 | 0.4 | No | No |
| SPAST | 6683 | M-014070-02 | 1.55 | 0.45 | Yes | No |
| TCHP | 84260 | M-014843-01 | 1.1 | 1.1 | No | No |
| APC | 324 | M-003869-01 | 0.45 | 0.96 | No | No |
| BAX | 581 | M-003308-03 | 0.25 | 0.2 | No | No |
| BAK1 | 578 | M-003305-02 | 1.0 | 0.25 | No | No |
| BCL2 | 596 | M-003307-06 | 0.3 | 0.5 | No | No |
| MAP1S | 55201 | M-016881-01 | 0.55 | 0.65 | No | No |
| ATG12 | 9140 | N/A | 2.55 | 0.15 | Yes | ND |
| STX17 | 55014 | 10620318/9 | 1.55 | 0.7 | No | ND |
| MEAN | - | N/A | 0.93 | 0.67 | N/A | N/A |
For the autophagy screen, entries in grey indicate partial phenotypes; ND: not determined; N/A: not applicable.