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. 2015 Jun 11;16(6):13427–13441. doi: 10.3390/ijms160613427

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Abundance and stability analysis of protein biomarker candidates by LCM–LRP. (A) Twelve protein biomarker candidates in brain tissue homogenates were verified and semi-quantified by triple-quadrupole mass spectrometry. The abundance of each protein was estimated by the peak area ratio of its corresponding unique peptide (n = 12); (B) Peak areas of 12 unique peptides in zone 1 were compared to those in zone 4 (1:4), and the relative peak area ratios (UP/UP) are shown (n = 12). * p < 0.05; ** p < 0.01.