Table 1.

Heat map of microRNAs differentially expressed between dogs in the three study groups (n = 6/group): normal dogs at risk of heart disease (Stage A), asymptomatic dogs with myxomatous mitral valve disease (MMVD) and mild to moderate cardiac enlargement (Stage B1/B2) and dogs with MMVD and congestive heart failure requiring multiple cardiac medications (Stage C/D).

miRNA Name	ANOVA		Stage B1/B2 vs. Stage A		Stage C/D vs. Stage A		Stage C/D vs. Stage B1/B2
	p Value	FDR a	p Value b	FC c	p Value	FC	p Value	FC
cfa-miR-302d	0.0010	0.0378	0.0050	−2.70	0.0053	−3.51	0.6970	−1.30
cfa-miR-380	0.0001	0.0119	0.0020	−2.48	<0.0001	−4.76	0.2020	−1.92
cfa-miR-874	0.0016	0.0434	0.0117	−2.37	0.0058	−3.69	0.2547	−1.56
cfa-miR-582	<0.0001	0.0004	0.0003	−2.28	<0.0001	−4.71	0.0159	−2.06
cfa-miR-490	0.0005	0.0261	0.0101	−2.24	0.0012	−4.03	0.0666	−1.80
cfa-miR-329b	0.0019	0.0490	0.0008	−1.96	0.0050	−2.36	0.8497	−1.20
cfa-miR-487b	0.0012	0.0427	0.0642	−1.45	0.0025	−3.08	0.0023	−2.12
cfa-miR-103	0.0002	0.0140	0.1719	1.29	0.0011	3.81	0.0031	2.96
cfa-miR-98	0.0014	0.0428	0.0090	2.15	0.0029	4.07	0.0341	1.90
cfa-let-7c	0.0003	0.0218	0.0309	2.28	0.0012	4.67	0.0080	2.05
cfa-let-7b	0.0006	0.0269	0.0123	2.38	0.0009	4.03	0.0342	1.69

^a False Discovery Rate; ^b Student’s t-test; ^c Fold change: negative numbers indicate decreases in expression, positive numbers for increases in expression. Red, green, and grey colors indicate a significant increase, decrease, and non-significance in expression, respectively.