Figure 3. ATP hydrolysis translocates tyrosine loops through movements of D2 domains.

(a) Side-view surface representation of Pex1/6^ATPγS and Pex1/6^ADP-AlFx. One heterodimer is omitted from the hexamer. One Pex1 D2 and Pex6 D2 domain of each complex is highlighted in red and blue, respectively. Underneath, a cartoon representation based on rigid body fits of homology models into negative stain EM maps is shown. Conserved aromatic residues Pex1^F771 and Pex6^Y805 are shown as green spheres. (b) Growth of strains expressing either wild-type (PEX1, PEX6), no (pex1Δ, pex6Δ) or mutated pex1, pex6 alleles with a modified pore loop (pex1^Y488A, pex1^H495A, pex1^F771A, pex6^Y528A, pex6^Y805A) or arginine finger residues (pex1^R855K, pex6^R607K, pex6^R611K, pex6^R892K) on either glucose or oleate as a single carbon source.