Table 2.
Spatial relationship of CTs, nuclear diameter, and volume within 3D resting and activated lymphocytes
| CTs | PHA Status | Intraprobe left CT | Intraprobe right CT | Interprobe closest | Interprobe furthest | Nuclear diameter | Nuclear volume |
|---|---|---|---|---|---|---|---|
| μm | μm | μm | μm | μm | μm3 | ||
| 1 – 13 | -PHA | 4.21 (1.07) | 4.79 (1.28) | 3.52 (1.75) | 5.70 (2.0) | 10.27 (0.98) | 996.17 (278) |
| 1 – 13 | +PHA | 10.32 (2.53) | 8.82 (4.31) | 5.48 (2.26) | 9.59 (2.98) | 17.15 (2.4) | 2922.92 (1083) |
| 2 – 4 | -PHA | 5.83 (2.06) | 5.99 (1.66) | 3.72 (1.57) | 5.89 (1.87) | 10.54 (0.88) | 964.10 (137) |
| 2 – 4 | +PHA | 10.42 (3.29) | 8.99 (2.78) | 5.01 (2.19) | 9.76 (3.22) | 16.65 (2.07) | 3171.36 (1003) |
| 15 – 3 | -PHA | 4.49 (1.11) | 5.47 (1.95) | 2.80 (1.42) | 5.11 (1.87) | 10.45 (1.02) | 893.98 (236) |
| 15 – 3 | +PHA | 8.62 (2.7) | 8.71 (2.81) | 4.00 (2.2) | 8.48 (2.63) | 17.23 (2.21) | 3126.29 (1034) |
| 6 – 5 | -PHA | 4.89 (1.64) | 6.11 (2.03) | 2.86 (1.28) | 5.13 (1.59) | 10.49 (1.11) | 815.17 (238) |
| 6 – 5 | +PHA | 8.74 (3.23) | 9.48 (2.67) | 4.31 (2.32) | 8.80 (2.92) | 15.93 (1.95) | 2831.50 (849) |
| 8 – 7 | -PHA | 5.36 (1.81) | 5.99 (1.71) | 2.92 (1.18) | 5.49 (1.90) | 11.18 (1.10) | 971.98 (216) |
| 8 – 7 | +PHA | 7.73 (3.47) | 9.45 (2.84) | 4.91 (2.66) | 8.32 (2.58) | 15.10 (2.25) | 2422.48 (984) |
| 10 – 9 | -PHA | 5.08 (1.82) | 5.65 (2.03) | 3.47 (1.59) | 5.58 (1.78) | 11.12 (0.88) | 997.48 (227) |
| 10 – 9 | +PHA | 8.79 (2.75) | 7.48 (2.65) | 4.31 (2.15) | 7.99 (2.72) | 15.89 (1.99) | 2789.34 (936) |
| 12 – 11 | -PHA | 4.87 (1.39) | 5.17 (1.83) | 2.81 (1.34) | 5.05 (1.64) | 10.62 (1.06) | 806.81 (183) |
| 12 – 11 | +PHA | 7.24 (2.67) | 8.95 (3.14) | 3.51 (2.12) | 7.87 (3.39) | 16.04 (2.41) | 2628.15 (914) |
| 14 – 16 | -PHA | 4.58 (1.52) | 4.55 (1.62) | 3.00 (1.59) | 4.45 (1.48) | 10.48 (0.99) | 758.17 (181) |
| 14 – 16 | +PHA | 7.83 (3.03) | 7.86 (2.04) | 4.50 (2.36) | 8.29 (3.03) | 16.40 (2.16) | 2945.85 (1034) |
| 17 – 22 | -PHA | 4.25 (1.52) | 3.78 (1.29) | 2.85 (1.06) | 4.32 (1.66) | 10.54 (1.07) | 706.02 (173) |
| 17 – 22 | +PHA | 5.88 (1.73) | 4.81 (1.23) | 3.95 (1.41) | 6.41 (3.11) | 13.14 (1.85) | 2040.62 (757) |
| 19 – 18 | -PHA | 3.26 (1.34) | 5.38 (2.44) | 3.40 (1.31) | 5.36 (1.53) | 10.48 (1.24) | 873.05 (295) |
| 19 – 18 | +PHA | 6.88 (1.71) | 8.63 (3.77) | 4.80 (2.66) | 8.75 (3.02) | 17.70 (2.3) | 3333.78 (1151) |
| 21 – 20 | -PHA | 3.60 (1.6) | 5.17 (2.33) | 2.39 (1.41) | 5.12 (2.07) | 10.98 (1.32) | 931.65 (361) |
| 21 – 20 | +PHA | 7.79 (3.2) | 9.26 (3.42) | 3.71 (2.03) | 8.05 (3.72) | 18.31 (2.61) | 3530.47 (1335) |
| X – Y | -PHA | - | - | 3.99 (1.72) | - | 10.51 (1.07) | 797.52 (224) |
| X – Y | +PHA | - | - | 8.75 (3.69) | - | 17.87 (1.84) | 4225.90 (972) |
| X | -PHA | 4.68 (1.83) | - | - | - | 10.67 (0.95) | 831.76 (197) |
| X | +PHA | 5.32 (2.19) | - | - | - | 10.76 (1.46) | 1292.68 (480) |
Provides an overview of all the 3D measurements obtained utilizing Imaris software (V7.6.3) for all 24 CTs in resting and activated lymphocytes. The CTs column indicates which CTs were probed together in the dual FISH experiment (e.g. 1 - 13 indicates chromosomes 1 and 13). PHA status indicates whether data is from resting (-PHA) or activated (+PHA) lymphocytes. The remaining columns present the average measurements in microns (μm or μm3) obtained from a minimum of 40 cells (20 per subject with the exception of the X–Y and X–X data, with 20 cells from the male and female subject evaluated, respectively). Fig. 2 provides examples of how each distance was measured. The standard deviation of the measurements in all studied cells is provided in parentheses. The intraprobe columns provides the measurement between homologous CTs, the first intraprobe column provides the distance between the first CT listed in the CT column (e.g. CT 1), with the second intraprobe column providing the distance between the homologous CT pair for the second CT in the CT column (e.g. CT 13). The two interprobe columns provides the distances between the two heterologous CTs (e.g. CT 1 and CT 13), the first interprobe column presents the distance for the two closest CTs, with the second column presenting the data for the two furthest CTs. Subsequently, both the nuclear diameter and volume is provided