Table 3.
Comparison of radial hierarchical organization of CTs between 2D and 3D methods
| CT hierarchy -PHA 2D (median) | CT hierarchy -PHA 3D (distance) | CT hierarchy + PHA 2D (median) | CT hierarchy + PHA 3D (distance) | |
|---|---|---|---|---|
| Nuclear Interior | 19 (1.78) | 19 (0.61) | 22 (1.99) | 22 (0.52) |
| 17 (1.85) | 22 (0.57) | 17 (2.00) | 19 (0.51) | |
| 22 (1.85) | 1 (0.50) | 19 (2.06) | 21 (0.48) | |
| 1 (2.08) | 15 (0.49) | 21 (2.14) | 17 (0.46) | |
| 21 (2.10) | 17 (0.49) | 10 (2.27) | 16 (0.42) | |
| 16 (2.12) | 16 (0.48) | 15 (2.29) | 1 (0.41) | |
| 15 (2.17) | 21 (0.46) | 8 (2.31) | 12 (0.41) | |
| 9 (2.23) | 20 (0.45) | 14 (2.34) | 15 (0.40) | |
| 14 (2.25) | 12 (0.44) | 16 (2.34) | 8 (0.40) | |
| 20 (2.30) | 14 (0.43) | 12 (2.37) | 10 (0.39) | |
| 8 (2.33) | X (0.43) | 6 (2.49) | 7 (0.39) | |
| 6 (2.34) | 8 (0.42) | X (2.53) | 20 (0.39) | |
| 12 (2.34) | 6 (0.42) | 1 (2.54) | 14 (0.39) | |
| 10 (2.38) | 11 (0.40) | 20 (2.63) | 6 (0.38) | |
| X (2.42) | 10 (0.40) | 4 (2.76) | 9 (0.38) | |
| 11 (2.49) | 9 (0.39) | 5 (2.85) | Y* (0.36) | |
| 4 (2.53) | 2 (0.37) | 9 (2.89) | 18 (0.35) | |
| 7 (2.58) | Y* (0.37) | 2 (2.96) | X (0.34) | |
| 5 (2.62) | 4 (0.37) | 11 (2.96) | 3 (0.34) | |
| 2 (2.69) | 7 (0.37) | Y* (2.98) | 13 (0.33) | |
| 13 (2.73) | 13 (0.34) | 3 (3.04) | 5 (0.33) | |
| 3 (2.77) | 3 (0.34) | 18 (3.04) | 4 (0.32) | |
| 18 (2.77) | 18 (0.32) | 13 (3.05) | 2 (0.32) | |
| Nuclear Periphery | Y* (2.83) | 5 (0.32) | 7 (3.12) | 11 (0.31) |
Presents the radial hierarchy for all 24 CTs in resting and activated lymphocytes using both 2D and 3D approaches from both subjects. CTs are ordered from the nuclear interior (top of the table) toward the nuclear periphery (bottom of the table). Following the CTs in parentheses are the numerical values used to order the CTs. In the 2D approach, CTs are ordered based on the midpoint of the distribution of the CT fluorescence across the five rings of equal area (median) (200 cells/CT). In the 3D approach, CTs are ordered based on their (distance) to the nearest nuclear edge following normalization against the radius of the nucleus to account for differences in nucleus size (40 cells/CT). Data for CT Y (Y*) is obtained from the male subject only (2D: 100 cells; 3D: 20 cells)