Fig. 5.

Reduced acetylated tubulin and increased phosphorylated neurofilament. a-d. Immunohistochemical analyses revealed the reduced level of acetylated tubulin in HSP27-S135F mice (b) compared to normal mice (a) and elevated level of phosphorylated neurofilament in HSP27-S135F mice (d) compared to normal mice (c). Scale bar = 100 μM. e. Western blotting from 3 mice from each group demonstrated the changes of the proteins level. f. The Western blotting image were quantitated. Data are mean ± standard deviation. *, p < 0.05; and **, p < 0.01