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. 2015 May 7;13:145. doi: 10.1186/s12967-015-0507-z

Figure 4.

Figure 4

Comparison of the in vitro suppression function of regulatory T cell subsets between G-BM and G-PB. Panels A-D. Representative flow cytometry plots of the proliferation of CFSE-labeled responder T cells in G-BM without (A) or with Treg Suppression Inspector (Miltenyi Biotec) co-cultured without (B) or with an increasing number of Treg cells at ratios of 4:1 (C), 2:1(D), and 1:1(E) of Tregs in G-BM. Panels F-H. Proliferation of CFSE-labeled responder T cells in G-PB with Treg Suppression Inspector (Miltenyi Biotec) co-cultured without (F) or with equal numbers of Treg cells from G-PB (G) or G-BM (H). Panel I. Suppression functions of Treg cells in inhibiting T-cell activation. G-BM-Treg indicates Tregs from G-BM, G-BM Tresp indicates Tresp from G-BM, G-PB-Treg indicates Tregs from G-PB, and G-PB Tresp indicates Tresp from G-PB (n = 5). *P < 0.05 by Wilcoxon signed-rank test (mean ± SD).