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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Int J Cardiol. 2010 Dec 15;156(2):133–138. doi: 10.1016/j.ijcard.2010.10.025

Figure 2.

Figure 2

Islet-1 is expressed during development of the human heart. (A) RT-PCR results for islet1 mRNA expression at CS18 (6 weeks pc) and F3 (11 weeks pc). Immunohistochemical analysis for islet-1 expressing cells, large numbers of isl+ cells are present in the OFT (B) and RA (C) at CS23 (8 weeks pc). Small numbers of isl+ cells are also present in ventricles at F6 (14 weeks pc) (D). Note the absence of staining in the no primary control, F6 (14 weeks pc) ventricle (E). Islet1 was visualised with 3’-diaminobenzidine (brown) and sections were counterstained with haematoxylin (blue) Magnifications size bar = 10μm for all images. Abbreviations: RT-PCR: reverse transcription–polymerase chain reaction, pc: post conception, Isl+: islet-1 positive, RA: right atria, OFT: outflow tract.