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. Author manuscript; available in PMC: 2015 Jul 3.
Published in final edited form as: Int J Cardiol. 2010 Dec 15;156(2):133–138. doi: 10.1016/j.ijcard.2010.10.025

Figure 3.

Figure 3

Representative results for co-expression analysis for ABCG2 and other stem, progenitor endothelial and cardiac markers. No cells expressed islet −1 and ABCG2 in the OFT (A) or RA (B) at CS23 (8 weeks pc). Rare cells that expressed both CD31 and ABCG2 are expressed in the embryonic heart at CS23 (8 weeks pc) in the RA, note a cell expressing ABCG2 alone and a dual labelled cell expressing both ABCG2 and CD31 (C). Small numbers of cells that express ABCG2 and CD34 are present in the developing heart, in the LV at CS23 (8 weeks pc) (D). Rare cells express both ABCG2 and alpha-actinin in the fetal heart at F1 (9weeks pc) (E). Images A,B.C, and E, ABCG2 visualised with 3’-diamionbenzidine (brown), islet-1, CD31 and alpha-actinin (sarcomeric) visualised with Vector blue (blue). Image D, ABCG2 visualised with Vector NovaRED (red), CD34 visualised with Vector SG blue/grey (grey). Magnifications: Size bar = 10μm Abbreviations: OFT: outflow tract, LV: left ventricle, pc: post conception, RA: right atria, LA: left atria, RV: right ventricle.