Table 3.
Taxonomy of Bacteria Recovered from within the Okarian Rover from Sample Sites A, B, and C (see Fig. 2) during the NWPDX-2009 Traverse from Kugluktuk to Cambridge Bay, Canada
| Taxonomy with 16S contigs in current studya | Strain No. current studyb | GenBank accession No. current study | Source locations for NCBI strains closest matchc | NCBI accession No. closest match | NCBI closest match | Number of isolates (sites) | R2A recovery temp. (°C) |
|---|---|---|---|---|---|---|---|
| Aeromicrobium tamlense | C1-37c-2 | JX517204 | dried seaweed, South Korea | DQ411541 | 0.983 | 1(C) | 37 |
| Arthrobacter agilis | A2-4c-5 | JX517205 | groundwater, South Korea | EU730943 | 0.987 | 1(A) | 4 |
| Arthrobacter flavus | A2-4c-14 | JX517206 | pond water, Antarctica | FR691390 | 0.991 | 1(A) | 4 |
| Arthrobacter sp. | A2-25c-5 | JX517207 | ice/snow, Antarctica; permafrost, Norway | DQ341415 | 0.986 | 2(A), 1(B) | 4, 25 |
| Arthrobacter sulfonivorans | A2-4c-15 | JX517208 | glacial sediments, Svalbard | FM955888 | 0.995 | 3(A) | 4 |
| Arthrobacter tumbae | C1-4c-3 | JX517209 | polar ices, location unknown | EU090712 | 0.999 | 1(A) | 4 |
| Bacillus amyloliquefaciens | B1-25c-5 | JX517210 | potato rhizosphere, Peru; soil, Brazil; soil, China; melon fruit, China; potassium mine, China; water, Hungary | JX036499 | 0.999 | 5(A), 6(B) | 25, 37 |
| Bacillus cereus | B2-37c-7 | JX517211 | marine sediment, Costa Rica | EU741083 | 1.000 | 2(B), 1(C) | 25, 37 |
| Bacillus circulans | C2-37c-4 | JX517212 | mosquito midgut, India | JN644554 | 0.999 | 1(A), 2(C) | 25, 37 |
| Bacillus clausii (B1512R) | A1-37c-4 | JX517213 | Belgium | AF329475 | 0.994 | 1(A) | 37 |
| Bacillus drentensis | A1-25c-6 | JX517214 | airline cabin, CA, USA; desert granite, AZ, USA | EU379279 | 0.996 | 2(A) | 25 |
| Bacillus firmus | A2-37c-11 | JX517215 | salt lake, China | GQ903397 | 0.999 | 1(A), 2(B) | 37 |
| Bacillus horikoshii | B2-37c-8 | JX517216 | lake water, India | GU001902 | 0.999 | 1(B) | 37 |
| Bacillus korlensis | A2-37c-4 | JX517217 | desert soil, China | EU603328 | 0.996 | 2(A) | 37 |
| Bacillus licheniformis (B1512R) | A1-25c-5 | JX517218 | fermented fish, China, India, and South Korea | JX025165 | 0.999 | 2(A), 3(B) | 25 |
| Bacillus megaterium | A1-25c-21 | JX517219 | JPL clean room, CA, USA; rhizosphere, China; desert granite, AZ, USA | AY030338 | 0.999 | 2(A), 2(B) | 25, 37 |
| Bacillus nealsonii | A2-37c-15 | JX517220 | plant debris, China | FJ544393 | 0.999 | 1(A), 1(B) | 37 |
| Bacillus niacini (B1512R) | A2-37c-23 | JX517221 | soil, India | GU339292 | 0.991 | 1(A) | 37 |
| Bacillus pumilus | A1-25c-19 | JX517223 | potassium mine, China; JPL and KSC clean rooms, USA | GU332600 | 0.999 | 2(A), 2(B), 2(C) | 25, 37 |
| Bacillus selenatarsenatis | A1-37c-12 | JX517224 | mushrooms, China | EU239470 | 0.995 | 2(A) | 25, 37 |
| Bacillus simplex | A1-25c-12 | JX517225 | plant leaves, roots, alpine grass, China; desert granite, AZ, USA; Arctic Ocean | FJ999940 | 0.999 | 4(A), 2(B) | 25, 37 |
| Bacillus sp. | A2-37c-20 | JX517226 | JPL clean room, CA, USA; ocean water, Japan; desert granite, AZ, USA; uranium mine, Germany | AY030333 | 0.998 | 4(A), 4(B), 3(C) | 25, 37 |
| Bacillus thuringiensis | A1-25c-14 | JX517227 | Jatropha endophyte, Singapore | JQ659733 | 1.000 | 3(A) | 25, 37 |
| Bacillus weihenstephanensis | A2-25c-6b | JX517228 | forest soil, France | CP000903 | 1.000 | 1(A) | 25 |
| Brevibacillus brevis | B1-37c-14 | JX517229 | plant compost, Spain | EF079071 | 0.998 | 1(B) | 37 |
| Brevibacillus borstelensis | A1-37c-13 | JX517230 | fermented soybean sauce, South Korea | FJ982663 | 0.999 | 2(A) | 37 |
| Brevibacterium sp. (B1512R) | A2-37c-14 | JX517231 | oil-contaminated soil, India | GQ865646 | 1.000 | 1(A) | 37 |
| Brevundimonas mediterranea | C2-25c-16 | JX517232 | Mediterranean Sea; airline cabin, USA | AJ244706 | 0.998 | 4(C) | 4, 25 |
| Brevundimonas bullata | B2-37c-16 | JX517233 | KSC spacecraft facility, FL, USA | EU977700 | 0.997 | 3(B), 1(C) | 25, 37 |
| Brevundimonas sp. | B2-25c-4 | JX517234 | swine effluent, USA | DQ337577 | 1.000 | 3(B) | 25 |
| Cellulomonas sp. | C1-25c-1 | JX517235 | soil, Taiwan | EU303275 | 0.990 | 1(C) | 25 |
| Citricoccus sp. | C2-25c-5 | JX517236 | seawater, Japan | AB594473 | 0.999 | 1(C) | 25 |
| Cryobacterium sp. | A2-4c-18 | JX517237 | lake sediment, Antarctica | GU244362 | 1.000 | 3(A), 2(B) | 4 |
| Dietzia maris | C2-4c-4 | JX517238 | Phoenix spacecraft, KSC, FL, USA | EU977748 | 0.992 | 7(C) | 4, 25 |
| Frigoribacterium sp. | B1-25c-3 | JX517239 | alpine plants, China; soil, Antarctica | DQ339618 | 0.997 | 1(A), 1(B) | 4, 25 |
| Georgenia soli | C1-25c-3 | JX517240 | iron-ore soil, India | FN356976 | 0.987 | 1(C) | 25 |
| Knoellia sinensis | A2-25c-9 | JX517241 | JPL clean room, CA, USA | AY167851 | 0.994 | 1(A) | 25 |
| Kocuria rosea | C2-37c-6 | JX517242 | KSC clean room, FL, USA; soil, Himalayas; cucumber leaves, China | JN084149 | 0.985 | 3(C) | 25, 37 |
| Leifsonia sp. | A2-25c-1 | JX517243 | oil shale semi-coke, Estonia | EF540446 | 0.993 | 1(A) | 25 |
| Lysinibacillus sphaericus | C2-37c-8 | JX517244 | cattle manure, Sweden; beach sand, Costa Rica | EU869258 | 0.999 | 3(A), 1(B), 1(C) | 25, 37 |
| Microbacterium oxydans | A2-4c-16 | JX517246 | barnyard soil, Finland | AM237353 | 0.987 | 3(A) | 4 |
| Microbacterium sp. | C2-25c-10 | JX517247 | glacial soil, China; raw milk, India; clinical specimen, Germany; environmental, China; oil shale semi-coke, Estonia; mountain snow, China; glacial meltwater, Mt. Everest | JQ946048 | 0.999 | 5(A), 7(C) | 25, 37 |
| Mycetocola reblochoni | C2-4c-9 | JX517249 | cheese, United Kingdom | DQ062100 | 0.976 | 1(C) | 4 |
| Mycetocola sp. | A2-25c-11 | JX517250 | location unknown | GU217690 | 0.982 | 1(A) | 25 |
| Nesterenkonia sp. | C1-25c-2 | JX517251 | soda lake sediments, China | GQ404473 | 0.989 | 1(C) | 25 |
| Paenibacillus amylolyticus | C2-37c-12 | JX517252 | forest soil, China; ryegrass rhizosphere, Switzerland | AM921628 | 0.980 | 1(B), 2(C) | 25, 37 |
| Paenibacillus contaminans | A1-25c-9 | JX517254 | lab bench, Taiwan | NR044325 | 0.988 | 1(A) | 25 |
| Paenibacillus glebae | C2-37c-1 | JX517255 | soil, Netherlands | AM745264 | 0.995 | 1(C) | 37 |
| Paenibacillus illinoisensis | A1-37c-14 | JX517256 | KSC clean room, FL, USA; garden soil, Russia | DQ870759 | 0.989 | 2(A) | 37 |
| Paenibacillus lautus | A1-25c-1 | JX517257 | Etruscan tomb and soil, Italy | EU249586 | 0.999 | 1(A), 2(B) | 25, 37 |
| Paenibacillus sp. | C2-4c-13 | JX517258 | plant debris, China; environmental sample, India | JN411476 | 0.997 | 1(A), 1(C) | 4, 37 |
| Paenibacillus polymyxa | A1-25c-13 | JX517259 | lake sediment, Italy | EU362611 | 1.000 | 1(A) | 25 |
| Paenibacillus ruminocola (B27F) | B2-37c-10 | JX517260 | cattle rumen, China | DQ085279 | 0.950 | 1(B) | 37 |
| Paenibacillus sp. | A2-37c-10 | JX517261 | JPL clean room, CA, USA; plants and soils in South Korea, China, and France; sawdust, Japan | EF690425 | 1.000 | 2(A), 2(B), 2(C) | 25, 37 |
| Paenibacillus terrigena | B2-37c-9 | JX517262 | cinnamon leaves, China | FJ174658 | 0.999 | 1(B) | 37 |
| Planococcus sp. | C2-4c-3 | JX517265 | snow, Tibet | HQ327129 | 0.999 | 3(C) | 4 |
| Pseudoclavibacter helvolus | B2-25c-12 | JX517266 | snow crab, Korea | HM584267 | 0.998 | 1(B) | 25 |
| Psychrobacillus psychrodurans | B1-25c-9 | JX517222 | marine brown alga, South Korea | EF101552 | 0.985 | 1(A), 1(B) | 25, 37 |
| Rhodococcus cercidiphylli | A2-25c-2 | JX517267 | plant leaves, China | EU325542 | 0.999 | 1(A) | 25 |
| Rhodococcus sp. | A2-25c-13 | JX517268 | deep ocean sediment, China | AY188941 | 0.993 | 1(A) | 25 |
| Rhodococcus globerulus | A2-4c-12 | JX517269 | potassium mine, China | GU332596 | 0.979 | 1(A) | 4 |
| Rummeliibacillus stabekisii | B1-37c-21 | JX517270 | KSC spacecraft facility, FL, USA | DQ870754 | 0.999 | 3(B) | 37 |
| Sanguibacter antarcticus | A2-25c-3 | JX517271 | beach sand, Antarctica | EF211071 | 0.993 | 1(A) | 25 |
| Solibacillus silvestris (B27F) | C2-37c-15 | JX517272 | plant leaves, India | JQ313581 | 1.000 | 1(C) | 37 |
| Sporosarcina aquimarina | A1-37c-1 | JX517273 | JPL clean room, CA, USA; seawater, South Korea | AY167819 | 0.988 | 2(A), 3(B), 1(C) | 25, 37 |
| Sporosarcina ginsengisoli | B1-37c-26 | JX517274 | ginseng soil, South Korea | AB245381 | 0.992 | 3(B) | 37 |
| Sporosarcina sp. | A2-4c-7 | JX517264 | alpine permafrost, China | JF778686 | 0.999 | 2(A) | 4 |
| Staphylococcus pasteuri | A2-37c-19 | JX517276 | soil, Iran | FR839669 | 0.999 | 1(A) | 37 |
| Terrabacter terrae | C2-4c-14 | JX517277 | soil, Spain | AY944176 | 0.945 | 1(C) | 4 |
Sixty-nine unique bacterial taxa were identified from 185 isolates recovered from interior samples in the Okarian rover.
a
Taxonomy was based on BLAST analysis with full contigs unless otherwise noted for situations in which only one primer sequence was used.
b
Isolate names represent bacteria from snow sites A, B, or C; sample locations at each site (1–6; see Fig. 3); temperature for lab incubation (4°C, 25°C, or 37°C); and strain number in sequence from 1.
c
AZ, Arizona; CA, California; FL, Florida; JPL, Jet Propulsion Laboratory; KSC, Kennedy Space Center; TX, Texas; USA, United States of America.