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Enzyme titrations of the six MLL/SET1-WRAD2 complexes comparing the activity of HeLa mono/di-nucleosomes (0.05 mg/mL, as DNA) to core histones (0.05 mg/mL) as substrates. Reactions were 1 h, 30°C, with 1 μM 3H-SAM, and the extents of methylation were determined with the scintillation/filter plate assay. (A–F) HeLa mono/di-nucleosomes (■); chicken core histone (□).
