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. 2015 Jul 3;15:134. doi: 10.1186/s12866-015-0454-0

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© Guldimann et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Immunofluorescence stained confocal images of bacteria in infected brain-slices. a Delineation of an infection focus (yellow line). L. monocytogenes are stained in red. The surface area covered by L. monocytogenes was drawn and calculated using the Fluoview software (Olympus FV10-ASW Version 03.01.01.09). Magnification 20×. b Representative double-immunofluorescence of a L. monocytogenes infected brain-slice. The vast majority of bacteria are found within microglia. Left: Microglia are stained with CD68 in green. Center: L. monocytogenes in red. Right: Overlay (bar = 40 μm)