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. 2015 Jun 1;35(6):431–440. doi: 10.1089/jir.2014.0127

FIG. 3.

FIG. 3.

IL-1β production induced by A. actinomycetemcomitans invasion is independent on caspase-1. (A) A. actinomycetemcomitans-invaded RAW 264 cells were cultured for the indicated times with or without pan-caspase inhibitor, Z-YVAD-FMK (100 μM) for 1 h before invasion. The secretion of IL-1β was determined by enzyme-linked immunosorbent assay (ELISA). Data are representative of 3 independent experiments performed in triplicate (*P<0.05, Student's t-test). (B) Detection of IL-1β protein by immunoblotting treated with Z-YVAD-FMK (100 μM). (C) RAW 264 cells were treated with siRNA against caspase-1. Detection of IL-1β protein by immunoblotting in Caspase-1 knockdown cells. (D) Cell viability was assessed in A. actinomycetemcomitans-invaded RAW 264 cells, caspase-1 inhibitor-treated RAW 264 cells, and caspase-1 knockdown RAW 264 cells by MTT assay. Data are representative of 3 independent experiments performed in triplicate (*P<0.05, Student's t-test). Inhibitor and knockdown of caspase-1 were confirmed by western blotting (lower panel).