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. 2015 Jun 1;35(6):431–440. doi: 10.1089/jir.2014.0127

FIG. 4.

FIG. 4.

A. actinomycetemcomitans invasion induces NLRP3 expression in RAW 264 cells. A. actinomycetemcomitans-invaded RAW 264.7 cells (MOI 50) were cultured for the indicated times. (A) Expression of the pattern recognition receptor gene, NLRP3, was determined by real-time RT-PCR at the indicated time points. Values are presented relative to noninvaded control cells, which were arbitrarily assigned a value of 1. Data are shown as the mean of 3 independent experiments, with 3 independent samples examined in each (*P<0.05, Student's t-test). (B) Detection of NLRP3 protein by immunoblotting. (C) A. actinomycetemcomitans-invaded RAW 264 cells were stained with FITC-anti-NLRP3, phalloidin, and mounted with DAPI-containing buffer. Blue, DAPI, nucleus; green, NLRP3; red, actin filament. Images (magnification×40) are representative of 3 independent experiments.