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. 2015 Jun 1;35(6):431–440. doi: 10.1089/jir.2014.0127

FIG. 5.

FIG. 5.

Role of NLRP3 in IL-1β secretion induced by A. actinomycetemcomitans invasion. RAW 264 cells were treated with siRNA against NLRP3. (A) Knockdown of NLRP3 was confirmed by real-time RT-PCR (upper panel) and western blotting (lower panel) in A. actinomycetemcomitans-invaded RAW 264 cells. (B) The expression of IL-1β protein in A. actinomycetemcomitans-invaded NLRP3 knockdown cells was detected by immunoblotting. (C) The secretion of IL-1β was determined by ELISA. Data are representative of 3 independent experiments performed in triplicate (*P<0.05, Student's t-test).