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. 2015 Jun 1;35(6):431–440. doi: 10.1089/jir.2014.0127

FIG. 6.

FIG. 6.

Involvement of cathepsin B in IL-1β secretion in A. actinomycetemcomitans-invaded RAW264 cells. (A) Detection of cathepsin B protein in A. actinomycetemcomitans-invaded RAW264 cells by immunoblotting. (B) RAW264 cells were pretreated with a cathepsin B inhibitor, CA074-Me for 2 h at the indicated concentrations before invasion. The secretion of IL-1β was determined by ELISA. Data are representative of 3 independent experiments performed in triplicate (*P<0.05, Student's t-test). (C) Detection of IL-1β protein by immunoblotting in A. actinomycetemcomitans-invaded RAW264 cells with CA074-Me pretreatment. (D) RAW264 cells were pretreated with CA074-Me (50 μM) for 2 h at indicated concentrations before invasion. The secretion of IL-1β was determined by ELISA. Data are representative of 3 independent experiments performed in triplicate (*P<0.05, Student's t-test).