Fig. 1.

Brain tumor-initiating cells (BTICs) are moderately susceptible to MyxV infection and killing. (A) The viability of a panel of BTICs was assessed using Alamar Blue at 48 hours following MyxV infection, with absorbance values normalized to the untreated control for each BTIC line. Data are presented as mean ± SEM (n = 3, each with 6 internal replicates). (B) BTICs demonstrate variable late viral gene expression following infection with 1 or 10 MOI MyxV-dsRED. Viral red fluorescent protein expression was assessed 24 hours post infection with MyxV-dsRED. Cells were imaged using a Zeiss Axiovert microscope at 100X magnification with a rhodamine filter (scale bar = 50 μm). (C) Whole-cell viral titers were performed to assess viral replication at 72 hours post infection, demonstrating productive infection of all BTIC lines by MyxV. Data represent the mean number of foci-forming units (FFU) at 72 hours post infection, with the input virus subtracted (n = 2).