Fig. 4.

Viral gene expression in combination-treated BT025 cultures increases over time and is enhanced by combination treatment with 5 of 6 synergistic compounds. (A) Brain tumor-initiating cells were infected with MyxV-FLuc with and without concomitant drug treatment, and luminescence was quantified at 0–72 hours post infection. Five drugs were shown to significantly increase (P < .05, *) viral luminescence at the 72 hour time point Data are plotted as the mean viability ± SEM of 3 independent experiments, each with 6 internal replicates. Data for all 11 drugs are shown in Supplementary Fig. S6. (B) Only pemetrexed increased whole cell virus titers at 72 hours post infection. Data are plotted as the mean viability ± SEM of 2 independent experiments, each with 2 internal replicates.