Skip to main content
. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Immunol. 2015 Jun 10;195(2):587–601. doi: 10.4049/jimmunol.1402545

FIGURE 5.

FIGURE 5

Peripheral expansion of Vβ6/8+NK1.1+ population of tetramer+ CD3+ T cells in Vα19i Tg mice. (A) Shows surface Vβ6/8.1-2 (X-axis) and NK1.1 (Y-axis) expression on tetramer+ CD4+ (top plots), DN (middle plots) or CD8+ (bottom plots) T cells in spleen and blood from uninfected Vα19iCα−/−MR1−/− (left panel) Vα19iCα−/−MR1+/+ (right panel) mice. Numbers in the upper right quadrants are NK1.1+ and Vβ6/8.1-2+ cells. (B) Increasing enrichment of tetramer+ NK1.1 lineage in Vβ6/8+ cells more than in Vβ6/8− subset from spleen to blood. (C) Vα19i Tg cells developing with MR1 have higher affinity for MR1/RL complexes. Shown in the left panel are Vβ6/8 (X-axis) vs. tetramer (Y-axis) FACS plots of splenic CD3+ MAIT cells from Vα19iCα−/−MR1−/−or Vα19iCα−/−MR1+/+ mice. Shown in the right panel are mean fluorescence intensities of CD3ε (top bar graph) and tetramer (bottom bar graph) staining of splenic tetramer+ Vβ6/8+NK1.1+ or Vβ6/8−NK1.1− CD3+ T cells from Vα19iCα−/−MR1+/+ or Vα19iCα−/−MR1−/− mice. Despite lower CD3 expression, Vα19i Tg T cells from MR1+/+ mice display higher affinity for MR1/RL tetramers than Vα19i Tg cells from MR1−/− mice. Furthermore, tetramer+ CD3+ MAIT cells expressing both Vβ6/8 and NK1.1 in Vα19i Tg MR1 sufficient mice display the highest affinity for tetramer binding. Data shown are from two separate experiments. P values are obtained by Mann-Whitney U-tests (n = 5/group) (**p<0.01 and ***p<0.001).