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. Author manuscript; available in PMC: 2016 Jul 15.

Published in final edited form as: J Immunol. 2015 Jun 10;195(2):587–601. doi: 10.4049/jimmunol.1402545

Vβ6/8 and NK1.1 expression by BAL tetramer⁺ CD3⁺ MAIT cells in Vα19iCα^−/−MR1^+/+ and B6-WT mice after mycobacterial infection. (A – C) Phenotype of tetramer⁺ CD3⁺ MAIT cell subsets in BAL fluids from infected Vα19iCα^−/−MR1^+/+ mice. (A) (Left column) Representative FACS plots from three separate experiments showing percentages of tetramer⁺ CD3⁺ Vα19i Tg T cell subsets. The numbers in the upper right quadrants indicate tetramer⁺ CD4⁺ (top plot), tetramer⁺ DN (middle plot) and tetramer⁺ CD8⁺ (bottom plot) MAIT cells. (Right column) Representative plots showing Vβ6/8.1-2 (X-axis) and NK1.1 (Y-axis) expression on tetramer⁺ CD4⁺ (top plot), DN (middle plot) or CD8⁺ (bottom plot) MAIT cells. The numbers in the quadrants indicate i) NK1.1⁻Vβ6/8.1-2⁻ tetramer⁺ cells (lower left quadrats), ii) NK1.1⁺ Vβ6/8.1-2⁻ tetramer⁺ cells (upper left quadrats), iii) NK1.1⁺Vβ6/8.1-2⁺ tetramer⁺ cells (upper right quadrats) and iv) NK1.1⁻ Vβ6/8.1-2⁺ tetramer⁺ cells (lower right quadrats). The NK1.1⁻ Vβ6/8⁻ tetramer⁺ and NK1.1⁺Vβ6/8⁺ cells were the most predominant subpopulations. (B) Representative data from three separate experiments showing percentage of CXCR3 (X-axis) and α4β1 (Y-axis) expression on NK1.1⁺Vβ6/8⁺ or NK1.1⁻Vβ6/8⁻ tetramer⁺ CD4⁺ (top plots), DN (middle plots) or CD8⁺ (bottom plots) MAIT cells in BAL from infected Vα19iCα^−/−MR1^+/+ mice. Numbers in the upper right quadrants are proportions of cells that expressed both CXCR3 and α4β1 integrin. (C) Representative data from three separate experiments showing AN of CXCR3 and α4β1-expressing NK1.1⁺Vβ6/8⁺ or NK1.1⁻ Vβ6/8⁻ tetramer⁺ CD4⁺ (top bar), DN (middle bar) or CD8⁺ (bottom bar) MAIT cells. (D – F) Frequency and phenotype of tetramer⁺ CD3⁺ non-Tg MAIT cell subsets in BAL fluids from infected B6-WT mice. (D) The pie chart shows the relative distribution of absolute numbers of tetramer⁺ CD3⁺ MAIT cell subsets in the BAL fluids from infected B6-WT mice. (E) (Left column) Representative FACS plots showing percentages of tetramer⁺ CD3⁺ MAIT cell subsets. Numbers in the upper right quadrants indicate tetramer⁺ CD4⁺ (top plot), tetramer⁺ DN (middle plot) and tetramer⁺ CD8⁺ (bottom plot) MAIT cells. (Right column) Representative FACS plots showing Vβ6/8 (X-axis) and NK1.1 (Y-axis) expression on tetramer⁺ CD3⁺ MAIT cell subsets. Numbers in the upper right quadrants are proportions of tetramer⁺ cells that expressed both Vβ6/8 and NK1.1, and (F) CXCR3 and α4β1 integrin expression on Vβ6/8⁺NK1.1⁺ tetramer⁺ MAIT cell population in the airways of infected B6-WT mice.