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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Immunol. 2015 Jun 10;195(2):706–716. doi: 10.4049/jimmunol.1403190

Figure 3. DM does not stabilize MHC II by preventing aggregation.

Figure 3

(A) Representative elution profiles of freshly purified DR1 monomer and DR1 loaded with HA (pre-incubation). The elution position and molecular mass of protein standards (unit: kilo Dalton) were shown above traces, with void volume Vo and total included volume VT indicated. Stabilization of (B) non peptide-loaded DR1 or (C) DR1 loaded with a weakly binding peptide W1A by DM or peptides was monitored by gel filtration (post-incubation). Black arrow on each plot indicated the aggregation of DR1. These traces are representative of at least four independent experiments.