Skip to main content
. Author manuscript; available in PMC: 2016 Jul 2.
Published in final edited form as: Mol Cell. 2015 Jun 11;59(1):133–138. doi: 10.1016/j.molcel.2015.05.021

Figure 2. Run-off transcription with modified promoters.

Figure 2

(A) Schematic illustration of modified promoters (also see Fig. S2). The wild type SNR20 promoter, the SNR20 promoter with a C+4G mutation introduced in the TSS (SNR20 91W), and the SNR20 promoter with deletions (indicated by dashed lines) bringing the +1 TSS to positions 29, 31, and 33 bp from the TATA box (SNR20 29D, SNR20 31D, SNR20 33D). (B) Run-off transcription with the modified SNR20 promoters. For reactions with the inhibitor-sensitive mutant TFIIK-L83G, 1.1 mM NA-PP1 was added.