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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Immunol. 2015 Jun 5;195(2):717–725. doi: 10.4049/jimmunol.1401250

Fig. 2. HY-specific iTreg attenuation of GVHD is antigen dependent.

Fig. 2

Male or female (B6 x bm12) F1 mice were lethally irradiated and transferred with 5 x 106 TCD-BM alone or plus 1–1.5 x 106/mouse CD25-depleted CD4+ T cells (Teffs) from B6 donors. HY-specific iTregs were generated and isolated as described in figure 1, were added at 0.5–0.75 x 106/mouse into donor graft at the same time of BMT. Overall survival (A) and body weight changes (B) are shown. The data are pooled from 3 replicate experiments with 10–15 mice in each group. In a separate experiment (n = 5–6), male (B6 x bm12) F1 mice were lethally irradiated and transferred with TCD-BM alone or plus 1 x 106/mouse HY-specific iTregs on day 0. CD25-depleted CD4+ T cells (Teffs) from B6 donors were injected at 2 x 106/mouse on day 3 after BMT. Recipient survival (C) and body weight changes (D) are shown. Asterisk indicates statistical significance: *p<0.05, **p<0.01, ***p<0.001