Male or female BALB.b mice were lethally irradiated and transferred with TCD-BM alone or plus 4 x 106/mouse HY-specific iTregs. On day 3, 25 x 106/mouse total splenocytes from normal B6 donors were injected into the recipients previously transferred with BM alone or BM plus iTregs. Recipient survival (A) and body weight changes (B) are shown. The data are pooled from 2 replicate experiments with 9–10 mice in each group. Male or female BDF1 mice were lethally irradiated and transferred with TCD-BM alone or plus at 4 x 106/mouse CD25-depleted total T cells from normal B6 donors. HY-specific iTregs were also included at 2 x 106/mouse into donor graft 3 days after BMT for some recipients. Recipient survival (C) and body weight changes (D) are shown. The experiments were done 2–3 times for each BMT model, but the data presented are from one experiment with 5–8 mice in each group using cell doses indicated. Seven days post Teff injection, BDF1 recipients as described in the legend were sacrificed and pathology samples of the skin, lung, liver, small and large intestine were collected (E) represents the pathology score of the various organs with 4 mice per group. Asterisk indicates statistical significance: *p<0.05, **p<0.01, ***p<0.001