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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Immunol. 2015 Jun 5;195(2):717–725. doi: 10.4049/jimmunol.1401250

Fig. 4. Stability and Efficiency of HY-specific iTregs.

Fig. 4

Lethally irradiated male or female (B6 x bm12) F1 mice were transplanted with B6 TCD-BM plus 1.5 x 106/mouse Teffs (CD4+CD25−) isolated from B6 Ly5.1+ mice. At the same time, 0.75 x 106/mouse HY-specific iTregs were also included into the donor graft to some recipients. Seven days after BMT, recipient spleen and liver were harvested and measured for expansion and infiltration of iTregs and Teffs. Mononuclear cells were isolated from recipient spleen (A) and liver (B), and expression of TCRVβ6 and Ly5.1 was shown in gated CD4+ live cells. The average numbers of total cells, Teffs (CD4+Ly5.1+) and iTregs (CD4+Vβ6+Ly5.1−) per mouse were shown in recipient spleen (C) and liver (D). Each group includes 3–4 mice, and the data represents 1 of 3 replicate experiments. Asterisk indicates statistical significance: *p<0.05, **p<0.01, ***p<0.001