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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Immunol. 2015 Jun 15;195(2):602–610. doi: 10.4049/jimmunol.1402835

Figure 2. Phenotype of vaccine elicited, HA-specific memory B cells.

Figure 2

The phenotype of HA-specific B cell populations was assessed by flow cytometry in PBMC samples collected 2wks post-H5N1 MIV boost (N=12, 2 subjects from each vaccine group). (A) Mean forward scatter area (FSC-A) as a marker of cell size of H5+, H1+ and H5+H1+ memory B cells relative to the parental IgG+ memory B cell population. (B) The relative mean fluorescence intensity (MFI) was calculated for surface markers CD19, CD20, CD21, CD22, CD24, CD27, CD38 and CXCR5 for each HA-specific B cell population (H5+, H1+ and H5+H1+) and normalised to the MFI of the parental IgG+ memory B cell population for each subject (illustrated as a dotted line at 1.0). * denotes p<0.05