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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Immunol. 2015 Jun 10;195(2):553–563. doi: 10.4049/jimmunol.1500627

Figure 1.

Figure 1

Eos expression by Treg and Tconv cells. (A) Eos mRNA expression by naíve and activated Treg and Tconv. Treg (CD4+Foxp3-GFP+) and Tconv (CD4+Foxp3-GFP−CD44lowCD62Lhigh) were FACS-sorted and activated with plate bound anti-CD3 and anti-CD28 for the indicated times. (B) Eos protein expression by Foxp3+Helios+, Foxp3+Helios− and Foxp3−Helios+ cells in different immune organs. (C) Eos protein expression by activated Tconv cells. Splenocytes were activated with plate-bound anti-CD3 and anti-CD28 (1 μg/well) for 72 h. (D) Eos protein expression on freshly explanted Helios− and Helios+ CD4+ and CD8+ T cells in 21 d old scurfy mice.