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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Immunol. 2015 Jun 1;195(2):507–518. doi: 10.4049/jimmunol.1500027

Figure 1.

Figure 1

Ablation of IL-13Rα1 leads to skewing of neonatal secondary immunity towards Th1 cells. (A) Newborn IL-13Rα1−/− or IL-13Rα1+/+ Balb/c mice were given Ig-OVA in saline, and 2 months later challenged with OVAp/CFA. The SP and LN cells pooled from 3 to 6 mice were stimulated with OVAp and IFNγ was measured by ELISA 24 hours later. Each bar represents the mean ± SD of triplicate wells from a representative of 3 experiments. (B) Newborn IL-13Rα1−/− or IL-13Rα1+/+ Balb/c hosts recipient of neonatal T cells from IL-13Rα1−/− or IL-13Rα1+/+ DO11.10 donors were given Ig-OVA (closed bars) or Ig-W (open bars) and later challenged with OVAp/CFA. The SP and LN cells were harvested on day 10 after challenge and stimulated with OVAp in vitro. IFNγ (left column) and IL-4 (right column) production by SP and LN cells were analyzed by ELISA. Each bar represents the mean ± SD of triplicate wells. This is representative of 5 experiments. (C) Shows compiled results from the 5 experiments carried out as described in (B). The bars represent the mean ± SE of the 5 experiments. The statistical analysis used student’s t test to compare results between donor T cells from IL-13Rα1−/− versus IL-13Rα1+/+ mice within both IL-13Rα1−/− and IL-13Rα1+/+ hosts.